Laser induced degradation of bacterial spores during micro-Raman spectroscopy

Micro-Raman spectroscopy combined with optical tweezers is a powerful method to analyze how the biochemical composition and molecular structures of individual biological objects change time. In this work we investigate laser induced effects in trapped object. Bacillus thuringiensis spores, which are robust organisms known for their resilience light, heat, chemicals used study. We trap spores monitor Raman peak from CaDPA (calcium dipicolinic acid), chemical protecting spore core. see correlation between amount power release spore. At 5 mW, water suspension remain intact over 90 min experiment, however, at higher powers an effect could be observed. SEM images exposed (after loss was confirmed) show notable alteration spores’ structure. Our data indicates that median dose exposure lose ?60 J 808 nm. For decontaminated/deactivated i.e., treated sodium hypochlorite or peracetic acid solutions, sensitivity on even more pronounced different behavior observed by two chemicals. Importantly, most likely photochemical since increase temperature order 0.1 K as suggested our numerical multiphysics model. results care must taken when using micro-Raman photoinduced may substantially affect results.